How to Use the NADPure Longevity Pen at Home
Beneath-the-skin NAD+ application. Five minutes. Once or twice a week. Everything you need to know — before, during, and after your first dose.
What Is Beneath-the-Skin Delivery — And Why It's the Right Method for NAD+
Beneath-the-skin delivery means depositing the compound into the fatty tissue layer that sits between your skin and your muscle. This layer is densely supplied with capillaries — small blood vessels that absorb compounds directly into the bloodstream, bypassing the digestive system entirely.
This is the same delivery route used for:
— Trusted at-home wellness delivery technology, used by millions worldwide
— Many biologics and hormone therapies
It is not experimental. It is one of the most widely used administration routes in the world.
For NAD+, the beneath-the-skin route solves a problem that oral supplements cannot: the gut absorption problem. When you swallow NAD+ or its precursors, digestive enzymes begin breaking them down before they can reach your cells. Read more about why oral NAD+ supplements don't work.
Beneath-the-skin delivery skips all of that. NAD+ absorbs directly into the tissue layer. That's where it needs to be.
How to Apply NAD+ at Home with the NADPure Pen
Prepare Your NAD+ Cartridge and Pen
Remove the NAD+ cartridge from its cold storage packaging. Inspect the solution — it should be clear to slightly yellow. No cloudiness or particles. Check the temperature indicator strip to confirm the cold chain was maintained during transit. Click the cartridge into the pen body until you feel it lock. Do not shake.
Choose and Prepare the Application Site
Three recommended sites: the abdomen (2 inches from your navel, either side), the front or outer upper thigh, or the outer upper arm. Choose a site. Using the alcohol swab, clean a 2-inch area around the chosen point. Let it dry completely — at least 30 seconds. Wet alcohol at the application point can cause a brief sting.
Deliver Your Dose Beneath the Skin
Gently pinch 1–2 inches of skin between your thumb and forefinger — this lifts the tissue layer away from the muscle below. Hold the pen at a 45-degree angle to the skin surface. Position the precision tip at the centre of the pinched area. Press the application button firmly and hold for 3 seconds. The full dose delivers in under 10 seconds. Release the pinch. You will feel minimal to no discomfort.
Remove the Pen and Dispose Safely
Remove the pen from the skin in the same 45-degree angle you used to insert it. Apply the alcohol swab to the site and press lightly for 5–10 seconds. Do not rub — this can cause bruising. Recap the pen (not the tip) and dispose of the used tip in a sharps container. Most EU pharmacies stock sharps containers; many will accept used containers for disposal.
NAD+ Application Sites: Abdomen, Thigh, and Upper Arm
Abdomen
The outer abdomen — at least 2 inches from the navel — is the most common beneath-the-skin application site. It offers consistent tissue depth in most adults, is easy to reach, and allows effective pinch-up. Avoid the area immediately around the navel and any area with active bruising or scar tissue.
Upper Thigh
The front or outer surface of the upper thigh (not the inner thigh) provides reliable tissue depth and is easily accessible in seated or lying positions. Some customers find this site slightly more sensitive — this varies by individual.
Upper Arm
The outer upper arm works well when the abdomen and thigh need rotation rest. It is slightly harder to access independently — lean against a wall or use a mirror. Ensure you are applying into fatty tissue, not muscle.
Rotation Protocol: Rotate application sites on a weekly basis. Applying repeatedly into the same site causes localised tissue changes (lipohypertrophy) that can reduce absorption. A simple rotation: abdomen Monday → thigh Thursday → arm the following Monday.
How Often Should You Use the NADPure Pen? Protocol Frequency Guide
The standard NADPure protocol is once or twice weekly. The clinical literature on beneath-the-skin NAD+ dosing supports a range of 25–100mg per session, twice weekly, as the maintenance protocol for adults over 40.
Week 1–2: Start with once weekly. Give your body time to assess tolerance. Mild side effects (brief flushing, application-site warmth, temporary fatigue the day of application) are normal and typically resolve within the first three to four sessions.
Week 3–4: If once-weekly is well-tolerated, transition to twice weekly. This is where most customers see meaningful, consistent results.
Maintenance: Most customers stabilise on twice-weekly dosing long-term. Some high-output users (athletes, executives with high cognitive demand) extend to three times weekly under their physician's guidance.
A note on dosing and your physician: NADPure is a supplement protocol reviewed by our scientific advisor. It is not a medicine. We encourage you to discuss your protocol with your doctor — particularly if you have active medical conditions, are on concurrent medications, or are considering higher-frequency dosing.
NADPure Pen vs IV Therapy vs Oral Supplements
Not all delivery methods are equal.
Oral Supplements
Broken down by gut enzymes before absorption. Blood NAD+ may rise; tissue translation is unclear. Daily dosing required.
- ✕ Rarely reaches tissue
- Cost: Low / low value
- ✕ No standard protocol
IV NAD+ Drip
Bypasses gut entirely. Effective — but requires 2–6 hours in a clinic. Effects described as transient. Costs €500–€1,500 per session.
- ✓ Reaches tissue
- Cost: €1,000–€2,000/month
- ✕ Requires clinic visit
NADPure Pen
Bypasses gut, avoids first-pass metabolism. Under 5 minutes at home. Cold-chain delivered. Full protocol included.
- ✓ Direct to tissue
- ✓ At home, 5 min
- ✓ Full protocol included
What to Expect: Week 1 Through Week 8
Your body rebuilds NAD+ reserves gradually. Here is what most customers experience on the standard twice-weekly protocol.
Getting comfortable. The application site may feel slightly warm after the first one to two sessions — this is normal. Some customers report a brief flushing sensation. Energy changes at this stage are subtle. Your body is beginning to rebuild NAD+ reserves at the cellular level.
First signals. Sleep quality is the first thing most customers report changing — deeper, more consistent, waking less at 3am. Some notice a steadier energy baseline by mid-afternoon — the familiar 3pm wall becoming less sharp.
Clarity returning. Cognitive sharpness is the most common feedback. Words that used to slip. Recall that used to lag. Customers describe it as “my baseline came back.” Recovery from exercise also begins improving.
Full protocol effect. The majority of customers on twice-weekly dosing report substantial improvement in energy, sleep, cognitive clarity, and recovery by the end of week 8. The protocol becomes a maintenance priority, not an experiment.
Results vary between individuals. These timelines are based on customer feedback and clinical literature on NAD+ restoration. They are not a guarantee. Your results depend on your baseline NAD+ depletion, age, concurrent health factors, and protocol adherence.
Safety, Contraindications, and When to Consult a Doctor
NAD+ beneath-the-skin application has a well-established safety profile in the published literature. Adverse events at standard doses are uncommon and typically mild.
Common (mild, self-resolving):
— Application site redness or warmth (first 1–2 sessions)
— Mild transient flushing
— Fatigue the day of application in first 2–4 weeks
— Nausea (rare; usually resolves after first few sessions)
Consult your physician before starting if you:
— Are pregnant or breastfeeding
— Are undergoing active cancer treatment
— Have known hypersensitivity to NAD+ or any cartridge components
— Are taking immunosuppressant medications
— Have a diagnosis of active autoimmune conditions
NAD+ and CD38: A note on cancer. Some early in vitro (cell culture) research suggested CD38 inhibition might affect certain cancer cell lines. This research does not translate to beneath-the-skin NAD+ delivery at the doses used in this protocol. However, out of an abundance of caution, we recommend customers with a current or recent cancer diagnosis discuss this protocol with their oncologist before starting.
Dr. Ivan Manolov, MD
The NADPure protocol was developed in collaboration with Dr. Ivan Manolov.
Common Questions About the Protocol
Using the NADPure longevity pen: load the cartridge, swab and prepare the application site (abdomen, thigh, or upper arm), lightly pinch the skin, position the pen at 45 degrees, and press the button. The precision tip delivers the dose in under 10 seconds. Total time under 5 minutes.
The three recommended sites are: the abdomen (2 inches from the navel), the upper thigh (outer or front), and the upper arm (outer fatty tissue). Rotate between sites weekly to prevent tissue buildup.
The standard protocol is once or twice weekly. Start at once weekly for the first 2–4 weeks to assess tolerance, then move to twice weekly for full protocol benefit.
Most customers report minimal to no discomfort. The pen uses a precision tip designed for comfort. The site may feel slightly warm for a few minutes after — this is normal.
The most common side effects are mild: brief site warmth or redness, occasional flushing in the first one to two sessions, and temporary fatigue the day of application. These typically resolve within 24 hours.
At-home precision delivery is trusted by millions worldwide for wellness routines. The NADPure protocol was developed with Dr. Ivan Manolov.
The actual dose takes under 10 seconds. Total preparation and administration time is typically 3 to 5 minutes.
The NADPure pen delivers NAD+ into the tissue layer just beneath the skin — the optimal zone for absorption. This method does not require clinical expertise, making it ideal for at-home use.
Yes. The abdomen — approximately 2 inches from the navel — is one of the three recommended sites. It offers reliable tissue beneath the skin in most adults and is the most common site for at-home use.
The NADPure pen uses a fine-gauge precision tip designed for beneath-the-skin delivery — similar to trusted at-home wellness pens — optimised for tissue depth and minimal discomfort.